Tanner G Hoog, Matthew R Pawlak, Benjamin F Bachan, Aaron E Engelhart Biochemistry and Biophysics Reports Volume 30, July 2022, 101238 Ions in the Hofmeister series exhibit varied effects on biopolymers. Those classed as kosmotropes generally stabilize secondary structure, and those classed as chaotropes generally […]
Author: Aaron Engelhart
[preprint] Parasites, infections and inoculation in synthetic minimal cells
Brock Cash, Nathaniel J. Gaut, Christopher Deich, Laura L. Johnson, Aaron E. Engelhart, Katarzyna P. Adamala https://www.biorxiv.org/content/10.1101/2022.05.11.491559v1.abstract Synthetic minimal cells provide a controllable and engineerable model for an increasing amount of biological processes. While much simpler than any live natural cell, synthetic cells offer a […]
[preprint] Expanding luciferase reporter systems for cell-free protein expression
Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E Engelhart, Katarzyna P Adamala https://www.biorxiv.org/content/10.1101/2022.05.10.491427v1.abstract Luciferases are often used as a sensitive, versatile reporter in cell-free transcription-translation (TXTL) systems, for research and practical applications such as engineering genetic parts, validating genetic circuits, and biosensor outputs. Currently, only […]
Akaby—Cell-free protein expression system for linear templates
Wakana Sato, Judee Sharon, Christopher Deich, Nathaniel Gaut, Brock Cash, Aaron E Engelhart, Katarzyna P Adamala PLoS ONE 17(4): e0266272. https://doi.org/10.1371/journal.pone.0266272 Cell-free protein expression is increasingly becoming popular for biotechnology, biomedical and research applications. Among cell-free systems, the most popular one is based on Escherichia […]
Switchable DNA-Based Peroxidases Controlled by a Chaotropic Ion
Tanner G Hoog, Matthew R Pawlak, Lauren M Aufdembrink, Benjamin R Bachan, Matthew B Galles, Nicholas B Bense, Katarzyna P Adamala, Aaron E Engelhart ChemBioChem Volume 23, Issue 9 e202200090 Here we demonstrate a switchable DNA electron‐transfer catalyst, enabled by selective destabilization of secondary structure […]
Programmable Fusion and Differentiation of Synthetic Minimal Cells
Nathaniel J Gaut, Jose Gomez-Garcia, Joseph M Heili, Brock Cash, Qiyuan Han, Aaron E Engelhart, Katarzyna P Adamala ACS Synth. Biol. 2022, 11, 2, 855–866 Synthetic cells can mimic the intricate complexities of live cells, while mitigating the level of noise that is present natural […]
[preprint] Controlled exchange of protein and nucleic acid signals from and between synthetic minimal cells
Joseph M Heili, Kaitlin Stokes, Nathaniel J Gaut, Christopher Deich, Jose Gomez-Garcia, Brock Cash, Matthew R Pawlak, Aaron E Engelhart, Katarzyna P Adamala https://www.biorxiv.org/content/10.1101/2022.01.03.474826v1.abstract Synthetic minimal cells are a class of small liposome bioreactors that have some, but not all functions of live cells. Here, […]
[preprint] T7Max transcription system
Christopher Deich, Brock Cash, Wakana Sato, Judee Sharon, Lauren Aufdembrink, Nathaniel J Gaut, Joseph Heili, Kaitlin Stokes, Aaron E Engelhart, Katarzyna P Adamala https://www.biorxiv.org/content/10.1101/2021.10.17.464727v1.abstract Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. Here we present a […]
Salty Environments: The importance of evaporites and brine environments as habitats and preservers of biosignatures
Scott Perl, Solmaz Adeli, Chhandak Basu, Bonnie K Baxter, Jeff Bowman, Eric Boyd, Morgan Cable, Aaron J Celestian, Charles S Cockell, Frank A Corsetti, Kate L Craft, Aaron Engelhart, Alberto G Fairen, Suniti Karunatillake, Kennda Lynch, Sally Potter-McIntyre, Frances Rivera-Hernandez, Mark Schneegurt, Susanne Schwenzer, Svetlana […]
Decoding RNA’s mysteries
Novel COVID-19 vaccines and some diagnostic tests use messenger ribonucleic acid (mRNA). Aaron Engelhart investigates ways to track the nucleic acid. CBS Website writeup on vaccines and diagnostics https://cbs.umn.edu/blogs/cbs-connect/decoding-rna%E2%80%99s-mysteries